HPLC Test

The HPLC blood test expanded as a High Performance Liquid Chromatography test, is performed to screen Thalassemias and Hemoglobinopathies. This test is performed by taking the sample mixture and separating it for further diagnosis in a medical laboratory. You can benefit from complete HPCL test Delhi, which helps identify the abnormal hemoglobin, Hb A2, Hb F, etc.

HPLC test is quite quick to show the results with high levels of accuracy and reliability in the results. There are multiple laboratories that charge various amounts for HPCL test price. HAXA Lab is a leading medical laboratory that is equipped with the latest technology required for proper HPCL blood test.

Some of the essential elements of chromatographs are high-performance pumps for delivering solvent at a constant flow rate and devices specifically designed for molecular separation called columns. The system formerly known as High Performance Liquid Chromatography was more often referred to as "LC '' as related technologies advanced. A high-speed analysis method known as Ultra High Performance Liquid Chromatography (UHPLC) has also grown in popularity in recent years.

The various forms of haemoglobin found in the blood are identified using haemoglobin (Hb) electrophoresis by HPLC. The red blood cells include the protein haemoglobin, which is responsible for carrying oxygen to the body's tissues and organs. This test is used to identify blood diseases, screen for hereditary problems, and assess how well blood disorder patients are responding to treatment. Additionally, it finds aberrant haemoglobin types that might obstruct the passage of oxygen.

Why is HPLC done?

As a component of a regular checkup

Identifying blood diseases

To keep track of the course of treatment for patients with abnormal haemoglobin

As a genetic disease screening test

What is measured by HPLC?

Different forms of haemoglobin in the blood are measured and identified by the haemoglobin (Hb) electrophoresis by HPLC test. Different forms of haemoglobin are found in the blood of humans. The amount of haemoglobin in the body changes with age. The protein found inside red blood cells is called haemoglobin. Its job is to carry oxygen to the body's tissues and organs.

The process of making haemoglobin is impacted when there is a mutation, a change in the DNA sequence. Hemoglobin exhibits anomalous behaviour as a result of these modifications, which also affects how normally healthy haemoglobin functions. As a result, less oxygen will need to be carried to tissues and organs.

This test uses a variety of haemoglobin types, including:

• Hemoglobin A - Adult hemoglobin
• Hemoglobin F

Newborns and developing foetuses both contain haemoglobin F. Hemoglobin A quickly takes its position after birth. Both adults and children have haemoglobin A. The most typical type of haemoglobin is that one. Rare aberrant haemoglobin types caused by genetic abnormalities include haemoglobin C, D, E, M, and S.

Types of HPLC columns

Normal Phase Columns

When the stationary phase is more polar than the mobile phase, normal phase columns are employed.

For instance, in normal phase chromatography, the less polar mobile phase might be hexane or similar organic solvent, and the polar stationary phase—also known as packing material—might be silica gel.

For samples containing tiny compounds, such as organic acids or medicines, this sort of column is frequently utilised. Biomolecules like glycosylated proteins can also employ it.

Reverse Phase Columns

When the stationary phase is less polar than the mobile phase, reverse phase columns are employed in reverse phase chromatography. To put it another way, reverse phase chromatography.

Acetonitrile, methanol, and tetrahydrofuran are frequent stationary phases, while water is frequently employed as the mobile phase (THF).

Compared to normal phase chromatography, this technique is more frequently utilised since it may be applied to a variety of analytical tasks.

Ion Exchange Columns

Ion exchange chromatography has a somewhat different method of column separation. This technique employs charge to separate chemicals that can be easily ionised rather than solely relying on polarity.

The mobile phase is often a polar aqueous buffer, such as salt water, while the stationary phase is typically an acid with either a positive or negative charge.

The attractive ionic interactions between the molecules in the sample and the charged stationary phase cause separation in this sort of column. As the sample goes through the column, the components will separate at various rates because each component will have a distinct level of attraction to the charged stationary phase.

Proteins, amino acids, and carbohydrates are typically separated using this technique.

Exclusion columns for sizes

Particle size is used in size exclusion chromatography to separate the material.

The stationary phase is porous, allowing only small particles to enter the pores while allowing bigger molecules to move through the column more quickly. Each molecule diffuses into the pores to a different amount, hence the size of the holes in the stationary phase controls the retention period and elution profile of each sample component.

For size exclusion chromatography, the stationary phase must have porous particles, which is why molecular sieves (like zeolites), polysaccharides, and polymers (like a conventional silica column) are most frequently utilized.

Proteins and carbohydrates are separated using this method.

We have an experienced certified, and well-trained staff that handles the entire process professionally and with complete precision. A blood sample is taken, which is further sent to the lab for evaluation and spotting of the disease. We also offer online home sample collection services for the ease of our customers. At HAXA Lab, you get to book full HPCL test Delhi at affordable rates. Our esteemed customer base is a testimony for our superior services.

HPLC BLOOD TEST IN DELHI

A hemoglobin blood test or HPLC test refers to the amount of hemoglobin that is present in your blood. Haemoglobin is an essential protein present in the blood that is responsible for transporting oxygen to the body organs and tissues. Haemoglobin also collects carbon dioxide from organs and tissues and brings it back to the lungs. 

Haemoglobin is composed of a proteinaceous part (known as globin) and a non-proteinaceous part (known as heme). Heme is composed of iron and a pigment known as porphyrin. The porphyrin pigment is responsible for imparting red color to the blood. Since hemoglobin plays a crucial role in the transport of oxygen to the blood, its low levels affect the survival of the cells due to lack of oxygen. Abnormal levels of hemoglobin indicate the chances of a blood disorder.

A HPLC blood test In Delhi can be advised by a doctor due to several reasons. A HPLC test can be a part of your complete blood count that is done during the routine medical examination. This is done to monitor your general health and to screen for disorders like anemia. Anemia is a condition wherein you have fewer red blood cells than the normal range. The HPLC blood test in Delhi is also conducted during the standard comprehensive newborn screening procedure. A hemoglobin test is also conducted if you are pregnant or planning a pregnancy.

HOW IS THE HAEMOGLOBIN TEST DONE?

The hemoglobin test or HPLC test is done on your blood sample. Thus, the doctor will collect your blood sample and conduct an experiment in the laboratory to evaluate the level of hemoglobin. To collect the blood sample, an antiseptic is applied to your arm and an elastic band is tied around the upper arm. This increases the blood flow to the veins making them swell. Thus, it is simpler for the doctor to find a vein and collect samples through a needle.

WHAT DOES THE HPLC TEST COST IN DELHI?

The hemoglobin test is quite affordable. The hemoglobin test price in Delhi at HAXA Labs is ₹599/- .We offer different discounts on various tests along with free sample collection from the comfort of your home.

HAXA Labs provides two ways for our patients to book an HPLC test in Delhi in a simple and hassle-free process. You can either book your test by calling our center, or you can also book it online from our official website from the comfort of your home.

You can also search for Full body health checkup near me, lipid test in Delhi, or RTPCR test near me so that you can get all the benefits of diagnostic centre and pathology lab in Delhi in the comfort of your home.